Biologists have learned to make blood cells delay healing

© RIA Novosti / barbarian Gertaera in photomacrography theBiologists have learned to make blood cells delay healing© RIA Novosti / barbarian Gertaera the image Bank

. Biologists from Canada discovered the hormone, added in the blood «preprogrammed» cells of the immune system and causes them to proceed with accelerated «fix» the damaged layers of the skin and other tissues, according to a paper published in the Journal of Investigative Dermatology.

«This therapy may be the salvation for diabetics – if it will work in their body, then we will be able to avoid amputation because of nonhealing wounds. In addition, it can help people with severe burns, accelerating healing and reducing the likelihood of infection of wounds during the recovery,» said Jean-Francois Calle (Jean-Francois Cailhier) at the University of Montreal (Canada).

The slightest damage to the skin in people suffering from diabetes, HIV and other diseases can cause a lot of trouble. They get the bacteria and there are non-healing ulcers, often forcing doctors to amputate the limbs of patients or perform other dangerous operations. Recently, scientists have discovered that these ulcers can be associated with the weakening of electropole cells, orchestrated their migration when the growth of the wound.

Calle and his colleagues have found a potential «conductors» of these migrations and transformations of cells, watching what was happening in the skin of mice. For these experiments, the researchers watched the cells accumulate in the open wound, removed some of them and watched, slow down or accelerate the tightening of the cut.

These observations helped scientists to identify two key components of the regeneration process of the RAS – protein MFG-E8 and related cells-macrophages – one of the main elements of the skin’s immune system, coordinating the work of dozens of other types of cells. Hit molecules MFG-E8 inside of macrophages, as found by the biologists, leads to «reprogramming» and causes them to trigger inflammation, and to suppress them and to promote tissue regeneration.

As shown by the first experiments, the input of this protein directly into the patient’s body can cause powerful side effects — there are scars and other blemishes too active repair of the skin. So scientists had to think about how to achieve the desired effect without harm to the body.

The solution to this problem, biologists have found stem cells in the blood. The researchers extracted some of these cells from the bone marrow of mice, turned them into macrophages and treated with a solution of MFG-E8, «reprogrammed» on a tightening of Russian Academy of Sciences. After that, the biologists covered the culture of these cells cuts on the body of mice and tracked whether there will be any changes in their rate of healing.

As the researchers note, accelerated wound healing was noticeable even to the naked eye – on the average, wounds in these rodents was delayed about two times faster and the speed of recovery was higher in those mice whose wound, scientists injected more cells.

Now biologists are working on adapting this technology for use in clinical trials on volunteers. In addition, they plan to conduct experiments on animals suffering from diabetes or burns, which will allow to understand whether the use of such cells and protein MFG-E8 to help people.